[ad#PhycoBiosciences AIM Interview]

Innovations

The quest for an edible, multipurpose, malarial vaccine

April 19, 2013
AlgaeIndustryMagazine.com

The scientists used a protein produced by the bacterium responsible for cholera, Vibrio cholerae, that binds to intestinal epithelial cells.

The scientists used a protein produced by the bacterium responsible for cholera, Vibrio cholerae, that binds to intestinal epithelial cells. Credit: Wikimedia

Can scientists rid malaria from the Third World by simply feeding algae genetically engineered with a vaccine? That’s the question biologists at UC San Diego sought to answer after they demonstrated last May that algae can be engineered to produce a vaccine that blocks malaria transmission. In a follow up study, just published online in the scientific journal Applied and Environmental Microbiology, they got their answer: Not yet, although the same method may work as a vaccine against a wide variety of viral and bacterial infections.

In their most recent study, which the authors made freely available on the Applied and Environmental Microbiology website at aem.asm.org, the researchers fused a protein that elicits an antibody response in mice against the organism that causes malaria, Plasmodium falciparum, which afflicts 225 million people worldwide, with a protein produced by the bacterium responsible for cholera, Vibrio cholerae, that binds to intestinal epithelial cells. They then genetically engineered algae to produce this two-protein combination, or “fusion protein,” freeze dried the algae and later fed the resulting green powder to mice. The researchers hypothesized that together these proteins might be an effective oral vaccine candidate when delivered using algae.

The result? The mice developed Immunoglobulin A (IgA) antibodies to both the malarial parasite protein and to a toxin produced by the cholera bacteria. Because IgA antibodies are produced in the gut and mucosal linings, they don’t protect against the malarial parasites, which are injected directly into the bloodstream by mosquitoes. But their study suggests that similar fusion proteins might protect against infectious diseases that affect mucosal linings using their edible freeze-dried algae.

Mosquitoes from the genus Anopheles transmit the protozoan that causes malaria

Mosquitoes from the genus Anopheles transmit the protozoan that causes malaria. Credit: Wikimedia

“Many bacterial and viral infections are caused by eating tainted food or water,” says Stephen Mayfield, a professor of biology at UC San Diego who headed the study. “So what this study shows is that you can get a really good immune response from a recombinant protein in algae that you feed to a mammal. In this case, it happens to be a mouse, but presumably it would also work in a human. That’s really encouraging for the potential for algae-based vaccines in the future.”

The scientists say bacterial infections caused by Salmonella, E. coli and other food and water-borne pathogens could be prevented in the future with inexpensive vaccines developed from algae that could be eaten rather than injected. “It might even be used to protect against cholera itself,” said James Gregory, a postdoctoral researcher in Mayfield’s lab and the first author of the paper. In his experiments with mice, he said, Immunoglobulin G (IgG) antibodies—which are found in blood and tissues—were produced against the cholera toxin, “but not the malaria antigen and we don’t quite understand why.”

Part of the difficulty in creating a vaccine against malaria is that it requires a system that can produce structurally complex proteins that resemble those made by the parasite, thus eliciting antibodies that disrupt malaria transmission. Most vaccines created by engineered bacteria are relatively simple proteins that stimulate the body’s immune system to produce antibodies against bacterial invaders.

Three years ago, a UC San Diego team of biologists headed by Mayfield, who is also the director of the San Diego Center for Algae Biotechnology, a research consortium seeking to develop transportation fuels from algae, published a landmark study demonstrating that many complex human therapeutic proteins, such as monoclonal antibodies and growth hormones, could be produced by the common algae Chlamydomonas. That got Gregory wondering if complex malarial transmission blocking vaccine candidates could also be produced by Chlamydomonas.  Two billion people live in malaria endemic regions, making the delivery of a malarial vaccine a costly and logistically difficult proposition, especially when that vaccine is expensive to produce. So the UC San Diego biologists set out to determine if this alga, an organism that can produce complex proteins very cheaply, could produce malaria proteins that would inhibit infections from malaria.

The edible algae Chlamydomonas, seen here at UC San Diego, can be grown in ponds anywhere in the world.

The edible algae Chlamydomonas, seen here at UC San Diego, can be grown in ponds anywhere in the world. Credit: SD-CAB

“It’s too costly to vaccinate two billion people using current technologies,” explained Mayfield. “Realistically, the only way a malaria vaccine will ever be used in the developing world is if it can be produced at a fraction of the cost of current vaccines. Algae have this potential because you can grow algae any place on the planet in ponds or even in bathtubs.”

Collaborating with Joseph Vinetz, a professor of medicine at UC San Diego and a leading expert in tropical diseases who has been working on developing vaccines against malaria, the researchers showed in their earlier study, published in the open access journal PLoS ONE last May that the proteins produced by the algae, when injected into laboratory mice, made antibodies that blocked malaria transmission from mosquitoes.

The next step was to see if they could immunize mice against malaria by simply feeding them genetically engineered algae. “We think getting oral vaccines in which you don’t have to purify the protein is the only way in which you can make medicines dramatically cheaper and make them available to the developing world,” says Mayfield. “The Holy Grail is to develop an orally delivered vaccine, and we predict that we may be able to do it in algae, and for about a penny a dose. Our algae-produced malarial vaccine works against malarial parasites in mice, but it needs to be injected into the bloodstream.”

“…bacterial infections caused by Salmonella, E. coli and other food and water-borne pathogens could be prevented in the future with inexpensive vaccines developed from algae…”

Although an edible malarial vaccine is not yet a reality, he adds, “This study shows that you can make a pretty fancy protein using algae, deliver it to the gut and get IgA antibodies that recognize that protein. Now we know we have a system that can deliver a complex protein to the right place and develop an immune response to provide protection.”

Mayfield is also co-director of the Center for Food & Fuel for the 21st Century, a new research unit that has brought together researchers from across the campus to develop renewable ways of improving the nation’s food, fuel, pharmaceutical and other bio-based industries and is this week hosting a major symposium on the subject at the Institute of the Americas at UC San Diego.

Two other researchers in Mayfield’s laboratory, Aaron Topol and David Doerner, participated in the research study, which was supported by grants from the San Diego Foundation, the California Energy Commission (500-10-039) and the National Science Foundation (CBET-1160184).

More Like This…

HOME Algae Industry Jobs

Copyright ©2010-2013 AlgaeIndustryMagazine.com. All rights reserved. Permission granted to reprint this article in its entirety. Must include copyright statement and live hyperlinks. Contact editorial@algaeindustrymagazine.com. A.I.M. accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

From The A.I.M. Archives

— Refresh Page for More Choices
Environmental Technology magazine notes that the difficulty in predicting how algae blooms will develop lies in their variform nature. With a multitude of different bloom...
Gerard de Souza reports for the Hindustan Times that researchers at the CSIR-National Institute of Oceanography Goa (India) have found a cleaner, cheaper method to grow b...
Researchers at Los Alamos National Laboratory and partner institutions have provided the first published report of algae using raw plants as a carbon energy source. The r...
Paris-based Solabia Group (“Solabia”) has acquired Algatech Ltd., a global leader in the development, cultivation and commercialization of ingredients delivered from micr...
Liu Jia reports for the Chinese Academy of Sciences that a “magic soil” made out of modified clays has proven effective in fighting red tide along China’s coastal waters ...
Israeli-based Algatechnologies, Ltd. (Algatech), is teaming up with the Italian R&D company, Sphera Encapsulation S.r.l (Sphera), to develop innovative functional ingredi...
Foodbev.com reports that French marine ingredients company Algaia will install a new specialty seaweed extract unit at its facility in Brittany, France, after securing €4...
Paul Brinkmann reports for UPI.com that Florida Atlantic University and three other research schools have launched studies this year to test people who live near the coas...
Judith Lewis Mernit writes in e360.yale.edu that an experiment being conducted by animal science professor Ermias Kebreab at the University of California, Davis, is testi...
Jason Huffman writes in UndercurrentNews.com that the Kampachi Company, a mariculture business focused on expanding the environmentally sound production of sashimi-grade ...
Bloomberg News reports that a newly approved Chinese drug for Alzheimer’s will start clinical trials in the U.S. and Europe this year as the country’s first novel therapy...
Steve Fountain writes in fortstocktonpioneer.com that, amid the 800-page law that last month set the country’s farm policy through 2023, is the expansion of federal suppo...