NCMA Algae Tips
Click here for more information about Liqofluxphenometrics515R1
Visit!Commercial Algae Professionals


Seeing the light — with a little help from algae optogenetics

February 21, 2016

Injecting viruses laden with DNA from light-sensitive algae into the eye of a legally blind person could let the patient see again.

Injecting viruses laden with DNA from light-sensitive algae into the eye of a legally blind person could let the patient see again.

kdropcapsatherine Bourzac writes in Technology Review that if all goes according to plan, sometime next month a surgeon in Texas will use a needle to inject viruses laden with DNA from light-sensitive algae into the eye of a legally blind person in a trial that could let the patient see again, if only in blurry black-and-white.

The study, sponsored by a startup called RetroSense Therapeutics, of Ann Arbor, Michigan, is expected to be the first human test of optogenetics, a technology developed in neuroscience labs that uses a combination of gene therapy and light to precisely control nerve cells.

The trial, to be carried out by doctors at the Retina Foundation of the Southwest, will involve as many as 15 patients with retinitis pigmentosa, a degenerative disease in which the specialized light-sensitive photoreceptor cells in the eye die, slowly causing blindness. The aim of the treatment is to engineer the DNA of different cells in the retina, called ganglion cells, so that they can respond to light instead, firing off signals to the brain.

The Texas study will be followed closely by neuroscientists who hope to eventually use optogenetics inside the human brain to also treat Parkinson’s or severe mental illness. “This is going to be a gold mine of information about doing optogenetics studies in humans,” says Antonello Bonci, a neuroscientist who is scientific director of the intramural research program at the National Institute on Drug Abuse in Baltimore.

Simple organization of the retina. Image courtesy of

Simple organization of the retina. Image courtesy of

Patients who have retinitis pigmentosa lose peripheral and night vision before eventually becoming blind. Candidates for the RetroSense study won’t be able to see much more than a hand moving in front of their face. RetroSense CEO Sean Ainsworth says he hopes that after the treatment patients will “see tables and chairs” or maybe read large letters.

Optogenetics was developed a decade ago in neuroscience labs as a way to precisely control the activity of nerve cells. It works by adding DNA instructions for a light-sensitive protein, channelrhodopsin, which algae use to sense sunlight and move toward it. Added to a nerve, it causes the cell to fire when exposed to a specific wavelength of light.

The technology is already helping scientists make rapid progress in understanding what brain cells underlie movement, motivation, pain, and many other basic brain functions in animals. In one experiment, Stanford University researchers led by Karl Deisseroth, one of the inventors of optogenetics, found they could switch the sensation of fear on and off in mice by shooting light through a fiber-optic cable at specific cells in their brains.

RetroSense was founded in 2009 to commercialize research carried out by Zhuo-Hua Pan, a Wayne State University vision expert who realized that the eye might be the easiest place to use optogenetics. Unlike the brain, the eye is transparent and sensitive to light, and it’s much easier to treat with gene therapy. No extra hardware or fiber-optic cables are needed, since light shines directly onto the retina.

The eye has two kinds of photoreceptor cells. Cones, named for their shape, are responsible for color vision. Rods respond to light at night. Both react to incoming photons by generating an electrical signal that is passed through a succession of nerve cells to the optic nerve and then to the brain.

To overcome the loss of photoreceptors, the strategy created by Dr. Pan and adopted by RetroSense works by injecting viruses containing algae DNA into the center of the eye. Their target is the topmost layer of cells in the retina, called ganglions. Once they start making the light-sensitive protein, the ganglion cells should fire in response to light.

Dr. Pan expects the treatment to generate at least 100,000 light-sensing cells in the retina. That could translate to substantial vision. So far, the only commercial technology to restore limited sight to blind people is an electrical implant called the Argus II that transmits video from a camera to a sheet of 60 electrodes stitched inside the retina, but it provides only a few pixels of visual information at a time.

Example of a Retinitis Pigmentosa (RP) symptom. RP is a group of inherited genetic disorders characterized by progressive peripheral vision loss and night vision difficulties followed by eventual central vision loss, and blindness in many cases.

Example of a Retinitis Pigmentosa (RP) symptom. RP is a group of inherited genetic disorders characterized by progressive peripheral vision loss and night vision difficulties followed by eventual central vision loss, and blindness in many cases.

The algae protein has some limitations. One is that it responds only to the blue component of natural light. As a result, RetroSense expects patients to experience monochromatic vision. Patients might perceive an object that doesn’t reflect any blue light at all as being black.

Speculation about what people will or won’t see – and what that subjective experience will be like – stems from results of studies on blind mice. Jens Duebel, who leads a group studying optogenetic vision restoration at the Institut de la Vision, in Paris, says that after treatment blind mice will move their heads to follow an image and also avoid a bright light when held in a dark box, just as healthy mice do.

Because the algae protein isn’t as sensitive to light as a normal retina, Dr. Duebel thinks patients might see in outdoor light but not very well indoors. Dr. Duebel is associated with GenSight Biologics of Paris, a company that developed a pair of goggle-mounted microprojectors it thinks could overcome that problem. The goggles convert a video feed into wavelengths of light that a genetically altered retina can respond to. The French company remains a few years away from starting a clinical trial of its technology, he says.

Other treatments using optogenetics are under development.

Read More

More Like This…

HOME A.I.M. Archives

Copyright ©2010-2016 All rights reserved. Permission required to reprint this article in its entirety. Must include copyright statement and live hyperlinks. Contact A.I.M. accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

From The A.I.M. Archives

— Refresh Page for More Choices
James “Jamie” Levine took over the reigns at Sapphire Energy in July of this year as former President and CEO Cynthia “CJ” Warner stepped down, retaining her role as chai...
Algix, parent company of Solaplast, will be inaugurating their algae-to-plastic facility in Meridian, Mississippi, on November 14, 2014. Solaplast's facility will be focu...
Developing renewable fuel from wet algae is one of the latest innovations Richland, Washington-based Pacific Northwest National Laboratory (PNNL) has successfully driven ...
Solazyme has announced that total revenue for the fourth quarter of 2014 was $14.5 million, compared with $11.3 million in the fourth quarter of 2013, an increase of 29%....
The vision of developing a community college degree program to train a high technology algae workforce was launched at New Mexico's Santa Fe Community College (SFCC) in 2...
Rich McEachran writes in the Guardian that, in the process of surfacing a road, layers of asphalt – which is composed mostly of bitumen (a byproduct of crude oil distilla...
Scientists have been investigating the likely future impact of changing environmental conditions on ocean phytoplankton, which forms the basis of all the oceans' food cha...
Jessie Rack reports for NPR that demand for plant protein of all types is growing in concert with the growing interest in the U.S. to reduce meat consumption. People, fro...
The demand for spirulina as a natural food colorant is robust in the North America food processing industry, according to a new study by market intelligence firm Future M...
A new $1 million relationship between Michigan State University and ExxonMobil will expand research in the fundamental science to advance algae-based fuels. Dr. David Kra...
Northwestern University researchers have developed a quantitative tool that might help bring back coral from the brink of extinction. The novel algorithm could help asses...
Tyler Treadway of TCPalm reports on technology joining the fight in response to the Florida algae blooms. He watches, as water from a boat basin topped with several inche...