go to http://www.aocs.org! Algaetech International — The Future is NowComplete Algae Monitoring System Visit  cricatalyst.com!Nexus — Leaders in Greenhouse Systems Integration

Process

Increasing algae’s productivity via light regulation

November 7, 2013
AlgaeIndustryMagazine.com

Photomicrograph of cyanobacterial mat from a California salt marsh. (Wayne Lanier/UC Berkeley)

Photomicrograph of cyanobacterial mat from a California salt marsh. (Wayne Lanier/UC Berkeley)

A“proof of concept” experiment described in the upcoming Dec. 2 issue of the journal Current Biology has shown that growing algae in constant light can dramatically boost the amount of valuable compounds that they can produce. The study found that when the biological clocks of cyanobacteria (blue-green algae) were stopped in their daylight setting, the amount of several biomolecules that they were genetically altered to produce increased by as much as 700 percent.

“We have shown that manipulating cyanobacteria’s clock genes can increase its production of commercially valuable biomolecules,” said Carl Johnson, Stevenson Professor of Biological Sciences at Vanderbilt University, who performed the study with collaborators at the J. Craig Venter Institute in Rockville, MD and Waseda University in Tokyo. “In the last 10 years, we have figured out how to stop the circadian clocks in most species of algae and in many higher plants as well, so the technique should have widespread applicability.”

Professor Carl Johnson, of Vanderbilt University

Professor Carl Johnson, of Vanderbilt University

In 2004, Johnson was a member of the team that determined the molecular structure of a circadian clock protein for the first time. Subsequent work mapped the entire clock mechanism in cyanobacteria, which is the simplest bioclock found in nature. The researchers discovered that the clock consisted of three proteins: KaiA, KaiB and KaiC. Detailed knowledge of the clock’s structure allowed them to determine how to switch the clock on and off.

In the current study, the researchers discovered that two components of the clock, KaiA and KaiC, act as switches that turn the cell’s daytime and nighttime genes on and off. They have dubbed this “yin-yang” regulation. When KaiA is up-regulated (produced in larger amounts) and KaiC is down-regulated (produced in smaller amounts), the 95 percent of the cell’s genes that are active during daylight are turned on, and the 5 percent of the cell’s genes that operate during the night are turned off. However, when KaiC is up-regulated and KaiA is down-regulated, then the day genes are turned off and the night genes are turned on.

“As a result, all we have to do to lock the biological clock into its daylight configuration is to genetically up-regulate the expression of KaiA, which is a simple manipulation in the genetically malleable cyanobacteria,” Johnson said.

To see what effects this capability has on the bacteria’s ability to produce commercially important compounds, the researchers inserted a gene for human insulin in some of the cyanobacteria cells, a gene for a fluorescent protein (luciferase) in other cells and a gene for hydrogenase, an enzyme that produces hydrogen gas, in yet others. They found that the cells with the locked clocks produced 200 percent more hydrogenase, 500 percent more insulin and 700 percent more luciferase when grown in constant light than they did when the genes were inserted in cells with normally functioning clocks.

Coauthors of the study include Research Associate Professor Yao Xu, Postdoctoral Fellow Ximing Qin and Graduate Student Jing Xiong from Vanderbilt; Assistant Professor Philip Weyman and Group Leader Qing Xu from the J. Craig Venter Institute in Rockville, Md., and Graduate Student Miki Umetani and Professor Hideo Iwasaki at Waseda University in Tokyo.

The research was funded by National Institute of General Medical Sciences grants GM067152 and GM088595, Department of Energy grant DE-FG36-05GO15027, Japanese Society for the Promotion of Science grants 23657138 and 23687002, the Asahi Glass Foundation and the Yoshida Scholarship Foundation.

Read More

More Like This…

HOME Algae Industry Jobs

Copyright ©2010-2013 AlgaeIndustryMagazine.com. All rights reserved. Permission granted to reprint this article in its entirety. Must include copyright statement and live hyperlinks. Contact editorial@algaeindustrymagazine.com. A.I.M. accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

From The A.I.M. Archives

— Refresh Page for More Choices
Scientists at the Energy Department’s National Renewable Energy Laboratory (NREL) have demonstrated that just two of six iron-sulfur-containing ferredoxins in a represent...
The University of Greenwich is leading a €10m international project, called the ‘D-Factory,’ to build a biorefinery to develop the microalga Dunaliella as a sustainable r...
Biomass abounds on Earth, as forests, fields, sewage and seaweed. But only a small fraction, mostly human or agricultural waste, can be harvested without posing environme...
Valensa International and Contract Biotics have announced that Contract Biotics has started construction of an additional six acres of algae production units at the compa...
Technical standards define critical terms and metrics to add wisdom for the algae industry. Agreement among science and business leaders represents possibly the most diff...
A series of articles by Stephen Mayfield and the UCSD Laboratory deserve recognition for their articles on algae-based medicines for malaria and cancer. Mayfield and his ...
Algenist®, Solazyme’s anti-aging skincare brand featuring microalgae, has announced its launch in Nordstrom locations throughout the United States. The launch into Nordst...
A team of six University of Calgary researchers has been awarded funding for their project, Cost Effective Biotechnology for Carbon Capture and Re-Use, based on the conce...
By sending algae into space, a U.S. Department of Agriculture (USDA) scientist and his team will be able to study some of the key mechanisms that control plant growth and...
The Algae Biomass Organization (ABO) released the following statement calling on the EPA to include Carbon Capture and Utilization strategies in rules proposed June 2, 20...
A new, outdoor system at the University of Dayton Research Institute has been producing a high volume of algae since its installation in the summer of 2013, even through ...
A University of New South Wales (UNSW)-led team of researchers has discovered how algae that survive in very low levels of light are able to switch on and off a weird qua...
Gilbert, AZ-based Heliae has announced a partnership with Sincere Corporation, a Japanese waste management and recycling company, to form a joint venture and develop a co...
Algae Industry Magazine is pleased to announce a new Algae 101 series by our popular blogger, Mark Edwards, Professor, Arizona State University. The Algae Solutions to Na...
Starting in the early 70s, agencies in the former USSR invested more than 20,000 person-years of research and development to produce Bio-Algae Concentrates (BAC) that hel...
Algae manufacturer Cyanotech Corporation has announced implementing three major initiatives to improve Astaxanthin production at their Kailua Kona, Hawaii-based cultivati...
Biofuels derived from the oils produced by algae may offer a low-cost sustainable alternative to fossil fuels. To achieve this goal, optimization of cost effective strate...
Perth, Western Australia-based Algae.Tec Limited has announced that the Reliance Group has converted the first tranche of options following the positive progress achieved...