Hot Products

GeneArt® Kits for Mutagenesis, Cloning and Assembly

June 28, 2012

GeneArt® Plus Kits from Life Technologies

GeneArt® Plus Kits from Life Technologies

Life Technologies Corporation has announced the launch of three next generation GeneArt® genetic engineering kits that allow molecular and synthetic biologists unprecedented speed, flexibility, precision, and efficiency for the seamless cloning, assembly, and editing of genetic material.

The GeneArt® Seamless PLUS Cloning and Assembly Kit allows investigators to complete complex assembly projects in days that would take weeks with typically available methods. The kits use a proprietary enzyme mix to recognize and precisely assemble DNA fragments without the need for restriction digestion, ligation, or introduction of extra DNA sequence (seamless). The kits are designed to work with any vector a researcher chooses plus 1 to 4 fragments (or more depending on fragment sizes and workflow) in an in vitro, typically < 30 minute room temperature reaction to create constructs up to 40kb in size.

Seamless cloning assembly

Seamless cloning assembly

The newly introduced GeneArt® Seamless Cloning and Assembly Enzyme Mix is ideal for creating constructs up to 13kb with the option for high-throughput assembly.

The GeneArt® Site-Directed Mutagenesis PLUS System can be employed to introduce deletions, insertions and substitutions ranging from small to large fragment sizes and can facilitate single or multi-site mutagenesis. Up to three sites can be edited in a single plasmid at greater than 90% efficiency. Up to 25 nucleotides can be altered in a single site, and results are delivered typically in less than three hours for plasmids up to 14 kb in size.

The new kits extend Life’s current market-leading product offerings in assembly and mutagenesis kits, the GeneArt® Seamless Cloning and Assembly Kit (for up to four fragments and constructs up to13kb), the GeneArt® High-Order Genetic Assembly Kit (a yeast-based system for up to 10 fragments and constructs up to 110kb), and the GeneArt® Site-Directed Mutagenesis System. With the new all-in-one enzyme/buffer mix and increased room temperature stability, the new kits offer increased flexibility, speed, and the option for high-throughput workflows. The new Mutagenesis PLUS system offers all of the single-site functionality of the current mutagenesis kits but with multi-site mutagenesis, the all-in-one enzyme/buffer mix, and increased room-temperature stability.

All products include access to Life Technologies’ free online GeneArt® Primer and Construct Design Tool for Seamless or High-Order Assembly and Mutagenesis: 
http://bioinfo.invitrogen.com/oligoDesigner/. This tool facilitates the in silico design, assembly, or mutagenesis of a DNA molecule using GeneArt® technology. The GeneArt® Design Tool:

  • Guides researchers through construct design, including fragment import, order, and orientation
  • Checks for areas of homology and potential design issues
  • Creates primers for pre-cloning or incorporating end-homology between fragments (if needed) or primers for mutagenesis, depending your workflow
  • Shows updated amino acid sequence for mutagenized constructs
  • Allows one-click online ordering for custom primers (not available in all countries)
  • Generates final construct sequence, in GenBank format, and map files for easy download or import into Vector NTI® software

More Like This…

Go to HOME Page

Copyright ©2010-2012 AlgaeIndustryMagazine.com. All rights reserved. Permission granted to reprint this article in its entirety. Must include copyright statement and live hyperlinks. Contact editorial@algaeindustrymagazine.com. A.I.M. accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

From The A.I.M. Archives

— Refresh Page for More Choices
Researchers at the Paul Scherer Institute (PSI) in Wädenswil, Switzerland, have succeeded in producing energy-rich gas from microalgae, and in doing so have demonstrated ...
U.S. farmers and biofuels makers are watching for the Environmental Protection Agency’s (EPA’s) final decision on the 2014 Renewable Fuel Standard rules, which will set t...
A team of Michigan State University algae researchers have discovered a cellular "snooze button" that has the potential to improve biofuel production and offer ...
Renewable fuels company Muradel has launched Australia’s first integrated demonstration plant to sustainably convert algae into green crude, as a first step towards a com...
Solazyme, Inc. and Versalis, the chemical subsidiary of Eni S.p.A., one of the world’s largest oil and gas companies, today announced a partnership to expand the commerci...
Cyanobacteria, also known as blue-green algae because of their color, have endured for more than 2.5 billion years, providing ample time to adapt to changes in the Earth'...
Tess Riley writes in TheGuardian.com about how spirulina may be able to combat malnutrition in developing countries. Spirulina is one of the oldest life forms on Earth, c...
Green Star Products, Inc. (GSPI) has signed a contract to build a demonstration facility in Las Vegas, Nevada, to produce commercial quality algae. The Hybrid Algae Produ...
Fort Myers, FL-based Algenol has announced that the U.S. Environmental Protection Agency (EPA) has approved fuels made from Algenol’s process as an advanced biofuel, meet...
In an age where customer input is as easy as a click, OriginOil has tapped directly into its intended market to R&D their next generation algae harvester -- with a de...
Researchers Greg O’Neil of Western Washington University and Chris Reddy of Woods Hole Oceanographic Institution (WHOI), have exploited an unusual and untapped class of c...
In a recent study, published in PLOS ONE Journal, the influence of light intensity on the growth and lipid productivity of Nannochloropsis salina was investigated in a fl...
Developing renewable fuel from wet algae is one of the latest innovations Richland, Washington-based Pacific Northwest National Laboratory (PNNL) has successfully driven ...
Kailua Kona, Hawaii-based Cyanotech has announced financial results for the third quarter and first nine months of fiscal year 2015, ended December 31, 2014. For the thir...
Solazyme has announced that total revenue for the fourth quarter of 2014 was $14.5 million, compared with $11.3 million in the fourth quarter of 2013, an increase of 29%....