twittertopbarlinks_eventstopbarlinks_requesttopbarlinks_archives
Click here for more information about Liqofluxphenometrics515R1
Visit cricatalyst.com!Readers Poll

Technology

GeneArt® Kits for Mutagenesis, Cloning and Assembly

June 28, 2012

GeneArt® Plus Kits from Life Technologies

GeneArt® Plus Kits from Life Technologies

Life Technologies Corporation has announced the launch of three next generation GeneArt® genetic engineering kits that allow molecular and synthetic biologists unprecedented speed, flexibility, precision, and efficiency for the seamless cloning, assembly, and editing of genetic material.

The GeneArt® Seamless PLUS Cloning and Assembly Kit allows investigators to complete complex assembly projects in days that would take weeks with typically available methods. The kits use a proprietary enzyme mix to recognize and precisely assemble DNA fragments without the need for restriction digestion, ligation, or introduction of extra DNA sequence (seamless). The kits are designed to work with any vector a researcher chooses plus 1 to 4 fragments (or more depending on fragment sizes and workflow) in an in vitro, typically < 30 minute room temperature reaction to create constructs up to 40kb in size.

Seamless cloning assembly

Seamless cloning assembly

The newly introduced GeneArt® Seamless Cloning and Assembly Enzyme Mix is ideal for creating constructs up to 13kb with the option for high-throughput assembly.

The GeneArt® Site-Directed Mutagenesis PLUS System can be employed to introduce deletions, insertions and substitutions ranging from small to large fragment sizes and can facilitate single or multi-site mutagenesis. Up to three sites can be edited in a single plasmid at greater than 90% efficiency. Up to 25 nucleotides can be altered in a single site, and results are delivered typically in less than three hours for plasmids up to 14 kb in size.

The new kits extend Life’s current market-leading product offerings in assembly and mutagenesis kits, the GeneArt® Seamless Cloning and Assembly Kit (for up to four fragments and constructs up to13kb), the GeneArt® High-Order Genetic Assembly Kit (a yeast-based system for up to 10 fragments and constructs up to 110kb), and the GeneArt® Site-Directed Mutagenesis System. With the new all-in-one enzyme/buffer mix and increased room temperature stability, the new kits offer increased flexibility, speed, and the option for high-throughput workflows. The new Mutagenesis PLUS system offers all of the single-site functionality of the current mutagenesis kits but with multi-site mutagenesis, the all-in-one enzyme/buffer mix, and increased room-temperature stability.

All products include access to Life Technologies’ free online GeneArt® Primer and Construct Design Tool for Seamless or High-Order Assembly and Mutagenesis: 
http://bioinfo.invitrogen.com/oligoDesigner/. This tool facilitates the in silico design, assembly, or mutagenesis of a DNA molecule using GeneArt® technology. The GeneArt® Design Tool:

  • Guides researchers through construct design, including fragment import, order, and orientation
  • Checks for areas of homology and potential design issues
  • Creates primers for pre-cloning or incorporating end-homology between fragments (if needed) or primers for mutagenesis, depending your workflow
  • Shows updated amino acid sequence for mutagenized constructs
  • Allows one-click online ordering for custom primers (not available in all countries)
  • Generates final construct sequence, in GenBank format, and map files for easy download or import into Vector NTI® software

More Like This…

HOME A.I.M. Archives

Copyright ©2010-2017 AlgaeIndustryMagazine.com. All rights reserved. Permission required to reprint this article in its entirety. Must include copyright statement and live hyperlinks. Contact editorial@algaeindustrymagazine.com. A.I.M. accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

Visit our 2017 International Reader’s Poll Platinum Sponsors

bigelow mbiolp_link sfcc

From The A.I.M. Archives

— Refresh Page for More Choices
Forbes is running an interview with Bren Smith, an Ashoka Fellow and the founder of GreenWave, an organization dedicated to restoring oceans, mitigating climate change an...
In one of the most comprehensive studies to date, University of North Carolina at Chapel Hill researchers have sequenced the genes of a harmful algal bloom, unveiling nev...
An unprecedented harmful algal bloom off the coast of New England this fall provided a unique opportunity for Waterville, Maine-based Colby College students studying at B...
Joy Lanzendorfer reports for NPR that, as seaweed continues to gain popularity for its nutritional benefits and culinary versatility, more people are taking up seaweed fo...
Fraunhofer-Gesellschaft reports in Science Daily that two algae species survived 16 months on the exterior of the International Space Station (ISS) despite extreme temper...
Essen, Germany-based Evonik, and Royal DSM, headquartered in Kaiseraugst, Switzerland, have announced their intention to establish a joint venture for omega-3 fatty acid ...