[ad#PhycoBiosciences AIM Interview]

Hot Products

GeneArt® Kits for Mutagenesis, Cloning and Assembly

June 28, 2012

GeneArt® Plus Kits from Life Technologies

GeneArt® Plus Kits from Life Technologies

Life Technologies Corporation has announced the launch of three next generation GeneArt® genetic engineering kits that allow molecular and synthetic biologists unprecedented speed, flexibility, precision, and efficiency for the seamless cloning, assembly, and editing of genetic material.

The GeneArt® Seamless PLUS Cloning and Assembly Kit allows investigators to complete complex assembly projects in days that would take weeks with typically available methods. The kits use a proprietary enzyme mix to recognize and precisely assemble DNA fragments without the need for restriction digestion, ligation, or introduction of extra DNA sequence (seamless). The kits are designed to work with any vector a researcher chooses plus 1 to 4 fragments (or more depending on fragment sizes and workflow) in an in vitro, typically < 30 minute room temperature reaction to create constructs up to 40kb in size.

Seamless cloning assembly

Seamless cloning assembly

The newly introduced GeneArt® Seamless Cloning and Assembly Enzyme Mix is ideal for creating constructs up to 13kb with the option for high-throughput assembly.

The GeneArt® Site-Directed Mutagenesis PLUS System can be employed to introduce deletions, insertions and substitutions ranging from small to large fragment sizes and can facilitate single or multi-site mutagenesis. Up to three sites can be edited in a single plasmid at greater than 90% efficiency. Up to 25 nucleotides can be altered in a single site, and results are delivered typically in less than three hours for plasmids up to 14 kb in size.

The new kits extend Life’s current market-leading product offerings in assembly and mutagenesis kits, the GeneArt® Seamless Cloning and Assembly Kit (for up to four fragments and constructs up to13kb), the GeneArt® High-Order Genetic Assembly Kit (a yeast-based system for up to 10 fragments and constructs up to 110kb), and the GeneArt® Site-Directed Mutagenesis System. With the new all-in-one enzyme/buffer mix and increased room temperature stability, the new kits offer increased flexibility, speed, and the option for high-throughput workflows. The new Mutagenesis PLUS system offers all of the single-site functionality of the current mutagenesis kits but with multi-site mutagenesis, the all-in-one enzyme/buffer mix, and increased room-temperature stability.

All products include access to Life Technologies’ free online GeneArt® Primer and Construct Design Tool for Seamless or High-Order Assembly and Mutagenesis: 
http://bioinfo.invitrogen.com/oligoDesigner/. This tool facilitates the in silico design, assembly, or mutagenesis of a DNA molecule using GeneArt® technology. The GeneArt® Design Tool:

  • Guides researchers through construct design, including fragment import, order, and orientation
  • Checks for areas of homology and potential design issues
  • Creates primers for pre-cloning or incorporating end-homology between fragments (if needed) or primers for mutagenesis, depending your workflow
  • Shows updated amino acid sequence for mutagenized constructs
  • Allows one-click online ordering for custom primers (not available in all countries)
  • Generates final construct sequence, in GenBank format, and map files for easy download or import into Vector NTI® software

More Like This…

Go to HOME Page

Copyright ©2010-2012 AlgaeIndustryMagazine.com. All rights reserved. Permission granted to reprint this article in its entirety. Must include copyright statement and live hyperlinks. Contact editorial@algaeindustrymagazine.com. A.I.M. accepts unsolicited manuscripts for consideration, and takes no responsibility for the validity of claims made in submitted editorial.

From The A.I.M. Archives

— Refresh Page for More Choices
Analia Murias 
reports for fis.com that Chilean exports of products made from macroalgae generated a total of $195 million US in the first seven months of 2014, according...
Most Americans get plenty of protein, primarily from animal products including meat, eggs and milk. But for many, ensuring a healthy protein intake can be challenging. In...
Solazyme has announced that total revenue for the fourth quarter of 2014 was $14.5 million, compared with $11.3 million in the fourth quarter of 2013, an increase of 29%....
Simris Alg, a pioneering agribusiness producing omega-3 from farmed algae, has been declared one of Sweden’s 33 hottest companies in new technology. The renowned list is ...
Tom Redmond and Yuko Takeo report for Bloomberg.com that, after 10 years of developing algae as a nutritional supplement generating $37.8 million in annual revenue, Japan...
Studies conducted by EnAlgae partners in Ireland, France and Belgium point the way to seaweed being a viable and sustainable feedstock for the future in North West Europe...
Using a newly devised technique, researchers at the Scottish Association for Marine Science (SAMS) have examined microalgae strains in the Culture Collection of Algae and...
Jessie Rack reports for NPR that demand for plant protein of all types is growing in concert with the growing interest in the U.S. to reduce meat consumption. People, fro...
A new $1 million relationship between Michigan State University and ExxonMobil will expand research in the fundamental science to advance algae-based fuels. Dr. David Kra...
Glass tubing manufacturer SCHOTT, and Algatechnologies Ltd. (Algatech), a commercial algae producer and one of the largest manufacturers of natural astaxanthin, have part...
The U.S. Department of Energy (DOE) has awarded Arizona State University (ASU) a three-year, $1 million grant to fund the Atmospheric Carbon Dioxide Capture and Membrane ...
CBS Miami reports that protesters are demanding answers and action over the toxic mess in Florida — a poisonous algae bloom plaguing four counties now under a state of em...